Application of immunoassay to microquantitation of environmental endocrine disruptors-dioxins-specific immunoassay

APPLICATION AND ADAPTATION OF DOT IMMUNOASSAY TO DETECT AND MEASURE RUBELLA VIRUS ANTIBODY

A dot immunobinding assay ( DIA) was used for a quantitative and qualitative assay of rubella antibody. Purified antigen and conjugated anti-human immunoglobulin (RAHlg) were prepared. Nitrocellulose paper dotted with the antigen was added to serially diluted sera or blood samples. The reacting antibodies were visualized by a peroxidase system. Development of a colored insoluble substrate ...

Environmental Thyroid Disruptors and Human Endocrine Health

In the last 30 years, there is increasing concern about chemical pollutants that have the ability to act as hormone mimics. Because of structural similarity with endogenous hormones, their ability to interact with hormone transport proteins, or their ability to disrupt hormone metabolism, these environmental chemicals have the potential mimic, or in some cases block, the effects of endogenous h...

Endocrine disruptors, environmental oxygen, epigenetics and pregnancy.

The placenta and its myriad functions are central to successful reproductive outcomes. These functions can be influenced by the environment encountered throughout pregnancy, thereby altering the appropriate genetic programming needed to allow for sustained pregnancy and appropriate fetal development. This altered programming may result from epigenetic alterations related to environmental exposu...

Environmental Endocrinology: Endocrine Disruptors and Endocrinopathies

APPLICATION OF DOT IMMUNOASSAY (DIA) FOR DETECTION AND TITRATION OF MEASLES VIRUS ANTIBODIES

Measles is one of the most contagious human diseases. Although mass vaccination programs have reduced the incidence of this disease, measles is still an important cause of morbidity and mortality among children in developing countries. Therefore the use of sensitive techniques to evaluate vaccine efficacy and level of immunity among members of susceptible communities is crucial. Serum neut...